New records of Sarcophagidae (Insecta: Diptera) collected in Cerrado fragments in the municipality of Campo Grande, Mato Grosso do Sul state, Brazil

arcophagidae or flesh flies (Diptera) have worldwide distribution and about 3,100 described species (PaPe et al. 2011). At least 800 species are known from Neotropical Region (PaPe 1996; Mello-Patiu et al. 2014). Flesh flies are mainly attracted to decaying organic material, feces, carrion and corpses, and their larvae breed on these substrates (Dias et al. 1984a; D’alMeiDa & liMa 1994; MelloPatiu et al. 2009). Many species are adapted to environments modified by human (D’alMeiDa 1983; Dias et al. 1984b) and are ecologically and forensically important as decomposers of decaying organic matter and as potential indicators of postmortem interval (oliveira-Costa et al. 2001). Some species are of medical and sanitary importance, either as vectors of human and animal diseases, or as producers of myiasis (GuiMarães et al. 1983).


EntomoBrasilis 13: e0873 (2020)
arcophagidae or flesh flies (Diptera) have worldwide distribution and about 3,100 described species (PaPe et al. 2011). At least 800 species are known from Neotropical Region (PaPe 1996;Mello-Patiu et al. 2014). Flesh flies are mainly attracted to decaying organic material, feces, carrion and corpses, and their larvae breed on these substrates (Dias et al. 1984a;D'alMeiDa & liMa 1994;Mello-Patiu et al. 2009). Many species are adapted to environments modified by human (D'alMeiDa 1983;Dias et al. 1984b) and are ecologically and forensically important as decomposers of decaying organic matter and as potential indicators of postmortem interval (oliveira-Costa et al. 2001). Some species are of medical and sanitary importance, either as vectors of human and animal diseases, or as producers of myiasis (GuiMarães et al. 1983).
The Sarcophagidae are divided into three subfamilies: Miltogramminae, Paramacronychiinae and Sarcophaginae. Miltogramminae are uncommon in South America, and most species are known as kleptoparasites of Hymenoptera. Most Paramacronychiinae are distributed in the non-tropical part of the Northern Hemisphere, while few species occur in the Southern Hemisphere, and only one species is endemic to the Neotropical Region. Some members of this subfamily are parasitoids or predators of other insects, and there are records of species bred from pulmonate snails and rotting tortoise eggs. Sarcophaginae is the largest subfamily in number of genera and species in the Neotropical Region. This subfamily is biologically diverse and exhibits a wide variety of life habits, including species that are saprophagous, necrophagous, coprophagous, parasitoids of insects, and predators of invertebrates. Some species can cause myiasis (PaPe 1996).
Sarcophagidae can usually be distinguish from all other Oestroidea by the following combination of characters: thorax with three longitudinal black stripes on the mesonotum, notopleuron with two or four bristles (two large and two small), meron with bristles, subscutellum weakly developed, abdomen checkered, abdominal sternites 1 and 2 exposed and overlapping the sides of the respective tergites in males (shewell 1987;Mello-Patiu et al. 2009). The external morphology of the Sarcophagidae is quite uniform or vary too much and few characters can be used safely for species identification. Identification is based mainly on the structures of the male terminalia (Carvalho & Mello-Patiu 2008; Mello-

e-ISSN 1983-0572
New records of Sarcophagidae (Insecta: Diptera) collected in Cerrado… Toma et al. (2020) systematics  Considering the absences of studies in Mato Grosso do Sul and the importance of checklists and inventories as support for several research areas, the aim of this study is to present an updated checklist of the Sarcophagidae species from Mato Grosso do Sul, in order to broaden the knowledge of the regional fauna.

MATERIAL AND METHODS
The municipality of Campo Grande has a total area of 8,118.4 km² and is located geographically in the central region of the state of Mato Grosso do Sul, Brazil ( Figure 1). According to the Köppen classification, the climate varies between Cfa (humid mesothermal subtype with no dry season) and the Aw (tropical wet and dry subtype with rainy summer and dry winter); most of the precipitation falls between October and April and low frequency of precipitation occur between June and August.
The present study was conducted in two fragments of Cerrado in the municipality of Campo Grande: a riparian forest remnant near the headquarters of the experimental farm of Embrapa Gado de Corte (20º27'S, 54º37' W -530 m) ( Figure 2)

RESULTS AND DISCUSSION
A total of 8,580 individuals (3,626 males and 4,952 females) were collected using traps baited with bovine liver, and 319 individuals (157 males and 162 females) were collected

e-ISSN 1983-0572
New records of Sarcophagidae (Insecta: Diptera) collected in Cerrado… Toma et al. (2020) with  (PaPe 1996). Prior to this study, there was no record of species of this genus from Mato Grosso do Sul. Blaesoxipha (A.) brazil and Blaesoxipha (T.) convena are the first recorded from this state. This genus has numerous parasitoid species, especially of Acrididae grasshoppers, Tenebrionidae beetles, but also of Mantidae, cockroaches and other Coleoptera and Saltatoria (PaPe & DahleM 2010). Some species of the subgenus Gigantotheca seem to breed in vertebrate and invertebrate carrions (PaPe 1996;PaPe & DahleM 2010). Blaesoxipha species can be recognized by the following combination of characters (PaPe 1996): postalar wall setose, trochanter of hind leg with posteromedian row of spines present in both sexes; male mid femur with ctenidium with normal spines; male terminalia with cercus bent backwards with cercal prong with spines dorsally, lateral styli fused to each other through a median plate-like structure proximal to the stylus, lateral styli collapsed with no outlet from sperm duct, and phallic vesica reduced or undeveloped.  1994,1996): Nearctic: United States (Florida, North Carolina, Oklahoma, Texas). Neotropical: Costa Rica, El Salvador. This is the first record of this species from Brazil. Its biology is little known.

Helicobia Coquillett, 1895
There are about 35 species of this genus worldwide, most of them recorded from Neotropical Region (PaPe 1996). Helicobia aurescens is the third species of this genus recorded from Mato Grosso do Sul, in addition to H. morionella and H. pilifera ). Species of this genus are recognized by their small size, large bristles mainly on thorax, ocellar and vertical bristles very strong, reclinate orbital bristles in both sexes, parafacial bristled, wing with vein R 1 setose and third costal sector bare, and male mid femur without ctenidium (PaPe 1996). These three species can be identified using redescriptions provided by loPes (1939)  . Helicobia aurescens has already been caught in traps baited with animal tissues (decomposing bovine liver and lung), dog feces, and domestic pig and rodent carcasses (Moura 2004;Mulieri et al. 2008;barros et al. 2008;Mulieri et al. 2010;Mello-Patiu et al. 2014;souza et al. 2015). In Curitiba, Paraná state, this species showed a preference for inhabited areas (Ferreira 1979). haired, male mid femur with ctenidium of flattened spines, tegula blackish, contrasting from orangish basicosta, male sternite 5 deeply cleft and usually with almost parallel sides, vesica elongated, phallus with lateral and medium styli. The identification of the Oxysarcodexia species is difficult, the species of this genus are mostly separate from each other by differences in the male terminalia, which in some cases have very similar morphology. Except for O. mineirensis, most of the species recorded from Mato Grosso do Sul can be identified using identification key to the species of Oxysarcodexia provided by loPes & tibana (1987), and a taxonomic synopsis provided by souza 2014.
Peckia pexata ( (Dias et al. 1984b;barros et al. 2008;rosa et al. 2011, valverDe-Castro et al. 2017. Its behavior has indicated necrophagous habits (Dias et al. 1984b;valverDe-Castro et al. 2017). This species was found in urban, rural and forest areas in Colombia, but was highly associated with the last two areas (valverDe-Castro et al. 2017), agreeing with the studies carried out by Dias et al. (1984a) in Belo Horizonte.